Health Expenditure, Poverty and Economic Development in Latin America 2000-2005

Two of the main problems of many Latin American countries are poverty and low levels of expenditure on health. In many countries there has been little progress in this regard due to the lack of resources, and thus it is important to focus on the main policies that may improve Latin American development because its positive effect on income per capita and health expenditure. This article analyses the evolution of health expenditure, poverty indicators and economic development in American countries for the period 2000-2005, and focus on the important positive effect that the necessary increase in industrial development will have on poverty eradication and health improvement for the next years. Both national policies and international cooperation to development in the poorest countries of Latin America should favor all the factors of production which contribute to foster economic development, and particularly the investment in industry.Health Expenditure, Education, Development, Poverty

Role of Membrane GM1 on Early Neuronal Membrane Actions of Aβ During Onset of Alzheimer\u27s Disease

The ability of beta-amyloid peptide (Aβ) to disrupt the plasma membrane through formation of pores and membrane breakage has been previously described. However, the molecular determinants for these effects are largely unknown. In this study, we examined if the association and subsequent membrane perforation induced by Aβ was dependent on GM1levels. Pretreatment of hippocampal neurons with D-PDMP decreased GM1 and Aβ clustering at the membrane (Aβ fluorescent-punctas/20 μm, control = 16.2 ± 1.1 vs. D-PDMP = 6.4 ± 0.4, p \u3c 0.001). Interestingly, membrane perforation with Aβ occurred with a slower time course when the GM1 content was diminished (time to establish perforated configuration (TEPC) (min): control = 7.8 ± 2 vs. low GM1 = 12.1 ± 0.5, p \u3c 0.01), suggesting that the presence of GM1 in the membrane can modulate the distribution and the membrane perforation by Aβ. On the other hand, increasing GM1 facilitated the membrane perforation (TEPC: control = 7.8 ± 2 vs. GM1 = 6.2 ± 1 min, p \u3c 0.05). Additionally, using Cholera Toxin Subunit-B (CTB) to block the interaction of Aβ with GM1 attenuated membrane perforation significantly. Furthermore, pretreatment with CTB decreased the membrane association of Aβ (fluorescent-punctas/20 μm, Aβ: control = 14.8 ± 2.5 vs. CTB = 8 ± 1.4, p \u3c 0.05), suggesting that GM1 also plays a role in both association of Aβ with the membrane and in perforation. In addition, blockade of the Aβ association with CTB inhibited synaptotoxicity. Taken together, our results strongly suggest that membrane lipid composition can affect the ability of Aβ to associate and subsequently perforate the plasma membrane thereby modulating its neurotoxicity in hippocampal neurons

Molecular Requirements for Ethanol Differential Allosteric Modulation of Ligand-Gated Ion Channels Based on Selective G Beta Gamma Modulation

It is now believed that the allosteric modulation produced by ethanol in glycine receptors (GlyRs) depends on alcohol binding to discrete sites within the protein structure. Thus, the differential ethanol sensitivity of diverse GlyR isoforms and mutants was explained by the presence of specific residues in putative alcohol pockets. Here, we demonstrate that ethanol sensitivity in two LGIC members, the GlyR adult alpha1 and embryonic alpha2 subunits, can be modified through selective mutations that rescued or impaired Gbetagamma modulation. Even though that both isoforms were able to physically interact with Gbetagamma, only the alpha1 GlyR was functionally modulated by Gbetagamma and pharmacological ethanol concentrations. Remarkably, the simultaneous switching of two transmembrane and a single extracellular residue in alpha2 GlyRs was enough to generate GlyRs modulated by Gbetagamma and low ethanol concentrations. Interestingly, while we found that these TM residues were different to those in the alcohol binding site, the extracellular residue was recently implicated in conformational changes important to generate a pre-open activated state that precedes ion channel gating. Thus, these results support the idea that the differential ethanol sensitivity of these two GlyR isoforms rests on conformational changes in transmembrane and extracellular residues within the ion channel structure rather than in differences in alcohol binding pockets. Our results describe the molecular basis for the differential ethanol sensitivity of two LGIC members based on selective Gbetagamma modulation and provide a new mechanistic framework for allosteric modulations of abuse drugs

Surface-peaked medium sensitivity of the optical potential: an exact result

Microscopic optical model potentials for elastic hadron-nucleus scattering usually take the form of a convolution of a two-body effective interaction with the target ground-state mixed density. Within the Brueckner-Bethe-Goldstone gmatrix approach for the effective interaction, nuclear medium effects are made explicit by means spatial integrals throughout the bulk of the nucleus. In this contribution we discuss a novel and exact approach to track down the manifestation of intrinsic nuclear medium effects. After examining the momentumand coordinate-space structure of a two-body effective interaction –spherically symmetric in its mean coordinate– it is demonstrated that the intrinsic medium effects in the optical potential depend solely on the gradient of a reduced interaction. This feature implies the confinement of intrinsic medium effects to regions where the density varies most, i.e. the nuclear surface. This finding may be of special significance in the study of nuclear collisions sensitive to the peripheric structure of nuclei. We illustrate some of its implications in the context of 10Be + p elastic scattering at 39.1A MeV

Seismic imaging and attribute analysis of Chicxulub Crater central sector, Yucatán Platform, Gulf of Mexico

Chicxulub Crater, formed ~66Ma ago by an asteroid impact on the southern Gulf of Mexico, is the best preserved of the three large multi-ring basins in the terrestrial record. The crater structure is characterized by a semi-circular concentric ring pattern, marking the crater basin, peak ring, terrace zone and basement uplift. Analysis of a grid of 19 seismic reflection profiles using seismic attributes, marker horizons, contour surfaces and 3-D views is used to investigate the stratigraphy of the central zone. We used interactive software and routine applications to map the impact breccias, breccia-carbonate contact and post-impact carbonates. Four horizons marked by high-amplitude reflectors representing high-impedance contrasts were identified and laterally correlated in the seismic images. Complex trace attribute analysis was applied for petrophysical characterization. Surface contour maps of base and top of stratigraphic packages were constructed, which mapped the impactites and post- and pre-impact carbonate stratigraphy. Basin floor, marked by the contact between the impact breccias and overlying carbonates is shown by laterally discontinuous high-amplitude reflectors. Discontinuous scattered reflectors interpreted as the upper breccias beneath the crater floor, have an average thickness of ~300msm. The Paleogene sedimentary units are characterized by multiple reflectors with lateral continuity, which contrast with the seismic response of underlying breccias. The basal Paleocene sediments follow the basin floor relief. Upwards in the section, the carbonate strata are characterized by horizontal reflectors, which are interrupted by a regional unconformity. Onlap/downlap packages over the unconformity record a period of sea level change

Seismic imaging and attribute analysis of Chicxulub Crater central sector, Yucatán Platform, Gulf of Mexico

Chicxulub Crater, formed ~66Ma ago by an asteroid impact on the southern Gulf of Mexico, is the best preserved of the three large multi-ring basins in the terrestrial record. The crater structure is characterized by a semi-circular concentric ring pattern, marking the crater basin, peak ring, terrace zone and basement uplift. Analysis of a grid of 19 seismic reflection profiles using seismic attributes, marker horizons, contour surfaces and 3-D views is used to investigate the stratigraphy of the central zone. We used interactive software and routine applications to map the impact breccias, breccia-carbonate contact and post-impact carbonates. Four horizons marked by high-amplitude reflectors representing high-impedance contrasts were identified and laterally correlated in the seismic  images. Complex trace attribute analysis was applied for petrophysical characterization. Surface contour maps of base and top of stratigraphic packages were constructed, which mapped the impactites and post- and pre-impact carbonate stratigraphy. Basin floor, marked by the contact between the impact breccias and overlying carbonates is shown by laterally discontinuous high-amplitude reflectors. Discontinuous scattered reflectors interpreted as the upper breccias beneath the crater floor, have an average thickness of ~300msm. The Paleogene sedimentary units are characterized by multiple reflectors with lateral continuity, which contrast with the seismic response of underlying breccias. The basal Paleocene sediments follow the basin floor relief. Upwards in the section, the carbonate strata are characterized by horizontal reflectors, which are interrupted by a regional unconformity. Onlap/downlap packages over the unconformity record a period of sea level change

Multi-epoch <i>L</i>-band Spectroscopy of the Be Star μ Centauri Prior to Outburst

With the aim of contributing to the understanding of the disk formation process in Be stars, we pursued a one-year spectroscopic observing campaign of the Be star μ Centauri in the L-band, using VLT/ISAAC. We present the nine near-IR spectra we obtained in an epoch of relative photometric quiescence prior to an outburst of ΔV = 0.4 magnitude. Visual estimates during the epoch of our L-band spectroscopy are also presented for the first time, together with the unpublished complete visual light curve between the years 1998 and 2014. We observe significant and monotonic changes in emission line strength of Bracket-α and Pfund-γ lines relative to Humphreys lines, and also in the continuum slope. We interpret these observed changes in terms of important changes to the column density of the line emitting regions, moving from an optically thin to an optically thick stage just prior to a major outburst. For each observing date, we provide estimates for the column density and relative extension of the line emitting region. If the changes observed toward the end of our observing campaign were related to mass-loss changes from the central star, they would correspond to an increase in a factor of two in the mass of the disk in the innermost region. If related to the visual outburst observed one month later, the variability observed in our spectra would be the first detection of the early disk formation process in the L-band.Instituto de Astrofísica de La PlataFacultad de Ciencias Astronómicas y Geofísica

A Multi-scale Biophysical Approach to Develop Structure-Property Relationships in Oral Biofilms

Over the last 5-10 years, optical coherence tomography (OCT) and atomic force microscopy (AFM) have been individually applied to monitor the morphological and mechanical properties of various single-species biofilms respectively. This investigation looked to combine OCT and AFM as a multi-scale approach to understand the role sucrose concentration and age play in the morphological and mechanical properties of oral, microcosm biofilms, in-vitro. Biofilms with low (0.1% w/v) and high (5% w/v) sucrose concentrations were grown on hydroxyapatite (HAP) discs from pooled human saliva and incubated for 3 and 5 days. Distinct mesoscale features of biofilms such as regions of low and high extracellular polymeric substances (EPS) were identified through observations made by OCT. Mechanical analysis revealed increasing sucrose concentration decreased Young's modulus and increased cantilever adhesion (p < 0.0001), relative to the biofilm. Increasing age was found to decrease adhesion only (p < 0.0001). This was due to mechanical interactions between the indenter and the biofilm increasing as a function of increased EPS content, due to increasing sucrose. An expected decrease in EPS cantilever contact decreased adhesion due to bacteria proliferation with biofilm age. The application OCT and AFM revealed new structure-property relationships in oral biofilms, unattainable if the techniques were used independently